High Throughput Analysis Laboratory
Mission
The NU-HTA provides researchers with equipment, advanced technology, and expertise for the development and execution of chemical screening for drug/biomarker discovery and high throughput (HTP) biological analysis. Instrumentation for plate based 96/384/1536 well liquid handling and assay detection available for walkup use for medium and high throughput assays.
Equipment Available
The NU-HTA features state-of-the-art laboratory automation equipment, including instruments for automated liquid handling and plate detection. All instruments are accessible to trained users on a 24/7 basis.
- Labcyte Echo550: Acoustic nanoliter liquid transfer
- TTP Labtech Mosquito: Contact nanoliter liquid transfer
- Tecan Fluent 1080: Automated liquid handler for complex liquid handling tasks
- Integra ViaFILL: Bulk dispenser with “GripTip” technology for low dead volume dispensing
- Tecan M1000 Multimode plate reader:- With onboard plate stacker
- Formulatrix Mantis: Low volume dispenser for precious samples
- BioTek Synergy Neo2 Multimode plate reader: Enabled for nanoBRET and
- Molecular Devices ImageXpress Confocal imager:- High throughput, high content cellular imaging
- BioRad 384CFX qPCR System: 384-well Real Time PCR
- Wavefront Biosciences Panoptic:- Fast kinetic fluorescence plate imaging system for GPCR and ion channel targets
- Agilent Bravo: Automated liquid handler for plate to plate transfers
- Nanion SyncroPatch 384:- Automated patch clamp system for high quality electrophysiological data acquisition and analysis
- Nanion FLEXcyte 96: Automated cardiac contractility measurements done in 96 wells in parallel
Services Offered
NU-HTA provides researchers access to innovative technology for large-scale screening and drug discovery. Levels of service range from walk-up instrument use to fully supported projects.
The HTA provides the following general services:- Drug discovery or biological screening
- Optimization and miniaturization of HTP assays
- Walk-up use of robotic liquid handling and detection equipment
- Grant preparation assistance for HTP projects
- Chemical and biological libraries
- High content imaging and analysis in both confocal and widefield modes
- Cell-based assays
- Promoter/reporter assays (GFP, Luciferase)
- Cell proliferation, viability, cytotoxicity
- Ion channel and GPCR
- Live cell/3D high content assays
- Enzymatic assays
- Kinase assays
- Coupled enzyme assays
- Protein binding assays
- Fluorescence polarization (FP)
- Fluorescence based thermal shift (FTS)
- AlphaScreen/AlphaLISA
- Nano-BiT and NanoBRET assays
- HTP molecular biology protocolsq
- PCR reactions in 384 well format
- Automated DNA,RNA or protein purification
- Large scale or complex liquid handling
- Crystallography tray preparation
- Tube to plate reformatting
- Sample concentration normalization
- Automated SPE sample prep
- NU-HTA manages small molecule compound libraries available for use as a screening resource.
- ChemBridge DIVERSet-CL (50,000)
- ChemDiv Kinase inhibitor set (20,000)
- ChemDiv Protein-protein interaction focused set (20,000)
- FDA-approved collection (1600)
- Clinical collection (1800)
- Bioactives and natural products library (2880)
- Cell Cycle/DNA Damage library (3250)
- Epigenetic focused library (300)
Acknowledgements
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
"This work was supported by the Northwestern University High Throughput Analysis Laboratory."